Hershey-Chase experiment (1952): [Alfred Hershey and Martha Chase]
Bacteriophages: Viruses that infects bacteria. They are made-up of proteins and DNA.
1. Bacteriophage with labeled Protein: The protein coat contains 35S.
2. Bacteriophage with labeled DNA: The protein coat contains 32P.
Using above two bacteriophage two separate experiments were conducted.
In the experiments following steps took place:
Step 1: Infection. The bacteriophage is mixed with bacteria
Step 2: Blending: Bacteriophage injects genetic material inside the cell.
Step 3: Centrifugation: Separation or Bacteria and Viruses
Step 4: Analysis of radioactive P and S.
Results:
1. Bacteriophage with labeled Protein - Radioactive seen in supernatant only. Protein did not enter the cell.
2. Bacteriophage with labeled DNA - Radioactive seen in Bacteria only. DNA entered the cell.
Conclusion:
DNA is the genetic Material!!!
