Observation and Results
|
|
1 |
2 |
3 |
4 |
Blank |
Test |
|
GOT / GPT substrate (ml) |
0.45 |
0.4 |
0.35 |
0.3 |
0.5 |
0.5 |
|
Std. Sodium pyruvate (ml) |
0.05 |
0.1 |
0.15 |
0.2 |
- |
- |
|
Distilled water (ml) |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
|
DNPH reagent (ml) |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
|
Mix thoroughly, keep at room temperature for 20 minutes. |
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|
0.4N NaOH. (ml) |
5 |
5 |
5 |
5 |
5 |
5 |
|
Karmen units (GOT) |
27 |
61 |
114 |
190 |
0 |
38 |
|
Karmen units (GPT) |
28 |
57 |
97 |
150 |
0 |
105 |
|
Mix and keep at room temperature for 10 minutes. Read absorbance at 530 nm. |
|
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|
A 530 GOT |
0.10 |
0.13 |
0.17 |
0.23 |
0.0 |
0.11 |
|
A 530 GPT |
0.05 |
0.11 |
0.17 |
0.19 |
0.0 |
0.15 |
Draw a curve of Karmen units on X axis versus absorbance on Y axis
|
A 530 GOT |
0.11 |
|
A 530 GPT |
0.15 |
