Recombinant DNA Technology

Genetic Engineering

Manipulation of genetic material material for a desired genetic change in predetermined way.

1. Can transfer genes between unrelated organism.

2. Engineered recombination helps in introduction of foreign genes.

-Peter Lobban and A. Dale Kaiser - Department of Biochemistry, Stanford University proposed recombinant DNA techniques.

-Stanley Cohen and Herbert Boyer (1972) Pioneered the rDNA technology

-Antibiotic resistance gene from Salmonella typhimurium

-Cloned in vector

-Introduced Anitbiotic resistance gene in E. coli