Isolating genomic DNA of a 'donor' .The cell or organism from which the required gene is taken is called 'donor'.
Fragmenting this DNA using "molecular scissors" (Enzymes).
Screening the fragments for a 'desired gene.'
Inserting the fragments with desired gene into a 'cloning vector'. ( a plasmid, cosmid, or phage DNA), so as to develop a recombinant DNA or chimeric DNA.
Introducing the recombinant vector into a competent host cell.
Culturing these cells to obtain multiple copies or clones of desired fragment of DNA.
Using these copies to 'Transform" suitable host cells so as to express the desired gene
