TYPES OF ELECTROPHORESIS

Electrophoresis can be divided into two main techniques:

1. Free electrophoresis or electrophoresis without stabilizing media

2. Zone electrophoresis or electrophoresis in stabilizing media.

1. FREE ELECTROPHORESIS

Free electrophoresis has two main techniques:

(i) Microelectrophoresis

(ii) Moving Boundary Electrophoresis.

Both the techniques have now become obsolete and are at best of historical Significance.

(i) Microelectrophoresis

This electrophoretic technique involves the observation of motion of small particles in an electric field with a microscope (such as R.B.Cs. neutrophils. bacteria etc.).

The suspension is contained in a closed system composed of a thin-walled section for optical observations and of suitable electrode compartments.

 

 

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